There Will Be One Folder With Data For Z Score Analysis: Biochemistry Research Paper, UCC, Ireland

1. IncuCyte Data Analysis:

You have received a portion of data to analyze today. In total, there will be 7 folders to analyze.

1. There will be one folder with data for z score analysis. You need to use those data for the calculation of the z score to validate an assay as feasible for the screening of compounds. There will be three folders.

2. Three folders are for an analysis of three biological repeats of experiment screening an effect of compounds on IRE1 activity in MDA-MB-231 cells (file name should include ‘not = stand for ‘no thapsigargin’). Check a plate map for treatments. I want to ask you to analyze data for DMSO, MKC8866 (or KIRA depends on the experiment), and compounds from 27 to 40. Please exclude from analysis compounds 1 – 26.

3. Three folders are for an analysis of three biological repeats of experiment screening an effect of compounds on IRE1 activity in MDA-MB-231 cells treated with thapsigargin. Check a plate map for treatments. I want to ask you to analyze data for DMSO, MKC8866 (or KIRA6 depends on the experiment), and compounds from 27 to 40. Please exclude from analysis compounds 1 – 26

-cell by cell analysis, calculate the percentage that expresses the reporter.

-DMSO= control

Analyze data, produce graphs, carry out statistical analysis to determine if the inhibitors worked, and discuss the results.

2. Bioinformatics Analysis of XBP1 protein involved in UPR

-Images of XBP1 protein on Chimera

– Draw a diagram of XBP1 in the IRE pathway involved in UPR and how it is involved in triple-negative breast cancer 

3. Literary review 1000 words on XBP1 in UPR

-Endoplasmic reticulum, UPR, XPBI, and IRE pathway and triple-negative breast cancer.